Protein Molecular Weight Marker “wide Range” (prestained) is ideal for precise sizing of proteins by SDS-PAGE and for Western blotting. Protein Marker “wide Range” 6,5 kD to 270 kD The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards supplemental data should be considered for more accurate adjustment in different electrophoresis conditions. This prestained protein ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. Under suggested conditions, GENE’s Protein Ladder resolves 10 major bands in 4-12% Bis-Tris Gel (MES buffer) and after Western blotting to the nitrocellulose membrane. This product was previously called Prism Ultra Protein Ladder (10 - 180 kDa). – 2.5 μl per well for general Western transferring.Ħ2.5 mM Tris-H 3PO 4 (pH 7.5 at 25 ☌), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN 3, 33% glycerol. The Bullseye Pre-stained Protein Ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa. – 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. D, single blot was split into two halves ( green line ) to be incubated with either rabbit anti-IDH1 antibody (ab172964 0.115 g/ml) or the corresponding rabbit monoclonal IgG isotype control (ab172730 0. The ladder is supplied in the gel loading buffer and is ready to use. Chameleon TM Duo pre-stained protein ladder for accurate sizing of protein bands. The Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Proteins are covalently coupled with a blue chromophore except for three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa) when separated on SDS-PAGE (Tris-glycine buffer). J Cell Sci 127:124-36 (2014).This Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa. Stripping buffer alters the chemiluminescent functionality. Entry into the nuclear pore complex is controlled by a cytoplasmic exclusion zone containing dynamic GLFG-repeat nucleoporin domains. Note: The WesternSure Ladder is not recommended for use when stripping and re-probing Western blots. Hypoxic 3D in vitro culture models reveal distinct resistance processes to TKIs in renal cancer cells. Isolation and characterizations of a novel recombinant scFv antibody against exotoxin A of Pseudomonas aeruginosa. Lactate regulates autophagy through ROS-mediated activation of ERK1/2/m-TOR/p-70S6K pathway in skeletal muscle. Effect of sulfur-containing agrochemicals on growth, yield, and protein content of soybeans (Glycine max (L.) Merr). Publishing research using ab116027? Please let us know so that we can cite the reference in this datasheet.Īb116027 has been referenced in 5 publications. Review other protein ladders in the unstained and prestained protein ladder guide. Recommended loading: ~2 - 3 µl for western blots.Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.Ready-to-use: Supplied in a loading buffer for direct loading on gels.The ladder is supplied in gel loading buffer and is ready to use.ĭo not heat, dilute, add reducing agent before loading. This product was previously called Prism Ultra Protein Ladder (10 - 180 kDa). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). Prestained Protein Ladder ab116027 is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa.
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